Journal: Journal of Neuroinflammation
Article Title: KChIP3 fosters neuroinflammation and synaptic dysfunction in the 5XFAD mouse model of Alzheimer’s disease
doi: 10.1186/s12974-025-03426-2
Figure Lengend Snippet: 5XFAD mice lacking KChIP3 (5XFAD/KChIP3 −/− ) increases microglia accumulation around βA plaques. Representative micrographs of βA plaques (Thioflavina-S, green) and microglia (Iba1, red) on ( a ) prefrontal cortex (CTX) and ( b ) dentate gyrus (DG) from WT, KChIP3 −/− , 5XFAD and 5XFAD/KChIP3 −/− . Magnification shows microglia accumulation around βA plaque on orthogonal views. Signal was captured using an Olympus FluoView 1000 inverted confocal multiphoton microscope. Scale bars: 100 μm 20X; and 50 μm in magnification 150X (60X objective lens with 2.5 digital zoom). Data were analyzed by unpaired T-test (*** p < 0.0001). n = 100 plaques from 2 male mice of 11 months old
Article Snippet: 40 well-stained pyramidal neurons from the CA1 region of the dorsal hippocampus (bregma –2.03 to –2.27 mm), [ ] of each experimental group (ten neurons per mouse) were identified and captured in an Olympus BX51-WI-DSU (Disk Scanning Unit) microscope (Olympus, Tokyo, Japan; Objective Microscope: 20x) coupled to the Stereoinvestigator 9 R software (MBF Bioscience, Williston, VT, US).
Techniques: Microscopy